| thursday october 25 - 02:24 |  FC Southampton [6] | 3-0 | Tranmere Rovers FC [3] |
| thursday october 25 - 06:22 | London United #14 [4] | 4-0 | Borneo Academy [0] |
| thursday october 25 - 08:39 | Chichester City [3] | 1-1 | Exmouth City [3] |
| thursday october 25 - 10:23 | Kettering United #2 [6] | 5-0 | Shrewsbury [0] |
| thursday october 25 - 11:29 | AFC Cumbria United [3] | 1-0 | Cramlington Town [3] |
| thursday october 25 - 17:32 | Eston and South Bank City [6] | 1-0 | Atherton City [4] |
| thursday october 25 - 17:45 | Minster United [3] | 3-3 | Skelmersdale City #2 [0] |
| thursday october 25 - 19:31 | Parkstone Lunatics [3] | 1-2 | Cambridge #6 [6] |
| thursday october 25 - 19:50 | Rushden United [3] | 1-0 | Brighton United #2 [1] |
| thursday october 25 - 20:38 | Watford City [1] | 1-1 | CrossFire FC [0] |
