| 星期二 五月 27 - 04:30 |  Clacton-on-Sea [4] | 1-1 | Jarrow Town [1] |
| 星期二 五月 27 - 08:47 | Peg Leg Pirates [3] | 0-0 | Guiseley City [1] |
| 星期二 五月 27 - 10:27 | Abbottishotspurs [6] | 1-2 | Stoke United #2 [3] |
| 星期二 五月 27 - 10:30 | Plymouth United #3 [1] | 1-1 | SuDoku AFC [1] |
| 星期二 五月 27 - 10:38 | CrossFire FC [2] | 2-0 | Norwich [1] |
| 星期二 五月 27 - 11:43 | Bury St Edmunds United [4] | 0-1 | Littlehampton City [4] |
| 星期二 五月 27 - 13:31 | Nottingham #3 [2] | 1-0 | North Shields United #2 [4] |
| 星期二 五月 27 - 13:41 | Dudley City [1] | 0-0 | Harrogate City [4] |
| 星期二 五月 27 - 19:43 | Camborne City [3] | 0-0 | Rugeley United [1] |
| 星期二 五月 27 - 20:20 | Clevedon United [3] | 0-0 | Hinckley United [2] |
