| thursday march 16 - 04:33 |  Longbenton [40] | 2-0 | London City #21 [41] |
| thursday march 16 - 05:30 | Southampton #5 [27] | 6-3 | South Shields FC [51] |
| thursday march 16 - 08:44 | Heswall Town #3 [66] | 4-3 | Leicester #4 [54] |
| thursday march 16 - 13:24 | Walsall [59] | 2-1 | Great Yarmouth United #2 [71] |
| thursday march 16 - 16:48 | Peterborough #3 [43] | 2-1 | High Wycombe United #2 [39] |
| thursday march 16 - 18:17 | Liverpool City #8 [50] | 3-0 | Newburn United #2 [45] |
| thursday march 16 - 18:40 | Walsall United #5 [61] | 4-2 | Fleet United #2 [47] |
| thursday march 16 - 19:20 | Herne Bay City [43] | 1-2 | Halifax City #2 [34] |
| thursday march 16 - 19:38 | Sunbury United #2 [74] | 1-1 | Eston and South Bank United #2 [48] |
| thursday march 16 - 19:49 | Leicester City #6 [26] | 2-1 | Stourbridge [60] |
