| tuesday september 5 - 02:20 |  Farnborough City #2 [3] | 0-2 | Northampton City #2 [7] |
| tuesday september 5 - 02:44 | Cheltenham #7 [2] | 4-1 | West Bromwich #2 [1] |
| tuesday september 5 - 08:39 | Swanley #2 [1] | 1-3 | Havant United #2 [6] |
| tuesday september 5 - 14:22 | Colchester United #4 [5] | 2-0 | Heanor [4] |
| tuesday september 5 - 14:47 | Middleton City [7] | 3-0 | Weston-super-Mare [6] |
| tuesday september 5 - 16:31 | Sheffield City #8 [6] | 3-2 | Leicester [9] |
| tuesday september 5 - 18:19 | Burnham-on-Sea United #2 [6] | 4-2 | Cheltenham City #6 [3] |
| tuesday september 5 - 18:29 | Sunderland #8 [0] | 0-1 | London City #10 [3] |
| tuesday september 5 - 19:36 | Rushden United #2 [1] | 5-1 | London United #26 [4] |
| tuesday september 5 - 19:50 | Camelot [5] | 2-0 | Bootle [4] |
