| monday january 22 - 02:26 |  Harlow United [27] | 1-2 | Birmingham Hunter [46] |
| monday january 22 - 02:40 | Huyton-with-Roby City [62] | 1-0 | Sunderland City #2 [59] |
| monday january 22 - 02:46 | We Lost [47] | 0-3 | Burton upon Trent City [53] |
| monday january 22 - 08:22 | Rushden United [13] | 0-1 | Northampton [50] |
| monday january 22 - 11:26 | Whitstable #2 [47] | 2-1 | Nilehorses United [83] |
| monday january 22 - 13:23 | Hyde [55] | 3-0 | London FC Legends [28] |
| monday january 22 - 16:18 | Hatfield Town [40] | 1-2 | Canvey Pixels [51] |
| monday january 22 - 17:30 | Fuchsia FC [56] | 1-0 | Canvey Island [67] |
| monday january 22 - 18:23 | Whitehaven Town [47] | 3-3 | Sunderland City [25] |
| monday january 22 - 18:35 | heavens light [50] | 2-0 | Qin Empire [58] |
