| wednesday june 26 - 02:30 |  Cheltenham #7 [50] | 1-2 | Durham United [63] |
| wednesday june 26 - 08:17 | Birmingham City #7 [40] | 0-1 | Failsworth City [49] |
| wednesday june 26 - 11:40 | Paignton United #2 [49] | 6-1 | Hertford United #2 [42] |
| wednesday june 26 - 11:49 | Wellingborough [28] | 1-3 | South Shields FC [41] |
| wednesday june 26 - 13:28 | Leeds City #3 [51] | 2-2 | Abingdon City #2 [40] |
| wednesday june 26 - 14:22 | Aylesbury United #2 [36] | 1-1 | Barnstaple City [45] |
| wednesday june 26 - 17:42 | Plymouth United [31] | 0-1 | Didcot [46] |
| wednesday june 26 - 19:21 | Borneo Academy [66] | 5-2 | Reigate [58] |
| wednesday june 26 - 20:22 | Peterlee United [45] | 2-1 | Woking United #3 [66] |
| wednesday june 26 - 20:35 | Bridlington [58] | 0-0 | Stoke #3 [54] |
