| tuesday august 31 - 04:45 |  Reading United #16 [4] | 1-1 | Highcliffe FC [7] |
| tuesday august 31 - 08:29 | Deal City #7 [5] | 1-5 | Farnworth United [9] |
| tuesday august 31 - 08:46 | Slough City #19 [1] | 1-2 | Bath City #4 [2] |
| tuesday august 31 - 10:23 | Leeds City #34 [0] | 0-0 | Chelmsford United #13 [4] |
| tuesday august 31 - 11:15 | Washington United #7 [3] | 0-0 | Canvey Island United #13 [9] |
| tuesday august 31 - 11:22 | London City #87 [1] | 1-0 | Loughborough City #7 [7] |
| tuesday august 31 - 14:33 | York #13 [6] | 1-2 | Workington United [4] |
| tuesday august 31 - 16:24 | Kirkby #4 [3] | 0-3 | Sutton Coldfield City #20 [4] |
| tuesday august 31 - 18:20 | Lowestoft #4 [0] | 3-0 | Farnborough City #7 [9] |
| tuesday august 31 - 20:33 | Peterlee United #7 [3] | 3-2 | Guiseley #7 [3] |
