| wednesday march 18 - 04:30 |  FC Trinec #4 [32] | 2-1 | FC Brno #8 [34] |
| wednesday march 18 - 05:31 | Ostrava Wild Beasts [42] | 1-2 | FC Union Cheb [60] |
| wednesday march 18 - 08:41 | FC Vyskov #2 [57] | 4-1 | FC Olomouc #5 [27] |
| wednesday march 18 - 14:37 | FK Karviná [26] | 0-1 | FK Prague #19 [22] |
| wednesday march 18 - 16:17 | FC Uherské Hradiste #3 [30] | 1-0 | FK Uherské Hradiste #2 [46] |
| wednesday march 18 - 20:31 | FK Prague #29 [45] | 1-1 | FK Jihlava #3 [16] |
| wednesday march 18 - 20:39 | FC Orlová #3 [51] | 6-0 | FK Písek #2 [43] |
| wednesday march 18 - 22:26 | FK Hodonín #2 [40] | 1-0 | FC Znojmo [46] |
