| wednesday september 1 - 06:46 |  Chabab Elhoguar [6] | 3-3 | ***** [3] |
| wednesday september 1 - 08:25 | FC STRATOVARIUS 1972 [0] | 0-3 | FK TÁBOR [3] |
| wednesday september 1 - 14:15 | FK Chomutov #2 [0] | 2-1 | FC Bohumín [4] |
| wednesday september 1 - 17:33 | FC Union Cheb [6] | 4-2 | FC Ostrau #7 [9] |
| wednesday september 1 - 19:25 | SK Prague [9] | 2-1 | SK Slavia Prague [9] |
| wednesday september 1 - 19:35 | Ostrava Wild Beasts [3] | 0-3 | FK Přestavlky [3] |
| wednesday september 1 - 19:35 | Sportklub Úpice [3] | 1-3 | FK Vořechov [9] |
| wednesday september 1 - 19:40 | AJAX Mistřice [1] | 0-4 | FK Košíře [3] |
