| tuesday may 23 - 09:48 |  New Addington [4] | 2-0 | SuDoku AFC [1] |
| tuesday may 23 - 10:46 | Kettering United #2 [3] | 2-3 | Gosforth United [4] |
| tuesday may 23 - 11:15 | Barnstaple City [4] | 2-2 | Reading United [4] |
| tuesday may 23 - 11:39 | London United #13 [3] | 3-1 | Potters Bar Town [3] |
| tuesday may 23 - 14:19 | Durham United [4] | 0-3 | Skelmersdale City #2 [2] |
| tuesday may 23 - 14:21 | Northampton City #2 [3] | 2-4 | Poole #3 [3] |
| tuesday may 23 - 19:28 | Cambridge #6 [3] | 4-0 | Stockton-on-Tees United #2 [1] |
| tuesday may 23 - 20:16 | Oxford United #2 [2] | 0-0 | Kidderminster United #3 [3] |
| tuesday may 23 - 20:19 | Coventry #6 [0] | 2-3 | Gloucester United #5 [3] |
| tuesday may 23 - 20:48 | Failsworth City [1] | 5-0 | Tranmere Rovers FC [3] |
